Now converted into one large space, these rooms like the preceding were once part of the ancient Medici theatre, and we can still see the original ceiling. It is one of the most famous parts of the Gallery, housing several Renaissance masterpieces that were produced in the last decades of the fifteenth century.

Of the fifteen works by Sandro Botticelli, the most famous are The Spring and The Birth of Venus, the first large paintings on profane subjects of the Italian Renaissance, which illustrate the cultural climate of Florence at the time of Lorenzo il Magnifico.

Botticelli's splendid sacred paintings are of an equally extraordinary intensity, such as the San Barnaba Altarpiece, or the exquisite medallions including the Madonna of the Magnificat.

One side of this vast room is occupied by a masterpiece of Flemish painting, the Portinari Triptych by Hugo van der Goes, a work which, since its arrival in Florence in 1483, exerted an enormous influence on the Florentine artists, starting with Botticelli himself.
The inspiration of Nordic culture also affected Domenico Ghirlandaio, three of whose paintings can be admired in the same room.